2019/20 Taught Postgraduate Module Catalogue

BIOL5178M High-Throughput Technologies

15 creditsClass Size: 60

Module manager: Professor David Westhead
Email: d.r.westhead@leeds.ac.uk

Taught: Semester 1 (Sep to Jan) View Timetable

Year running 2019/20

Pre-requisite qualifications

Biochemistry and molecular biology to undergraduate level.

Module replaces

BIOL5274M High throughput Technologies (10 credits)

This module is not approved as an Elective

Module summary

The module aims to provide an understanding of the use of high-throughput biomolecular data generation methods. The emphasis will be on understanding methods and the data that they typically give. Data analysis methods will be discussed but there will not be a strong emphasis on statistical methods that are covered in another module. Some experience of data analysis will be provided.

Objectives

The objectives of the module are to:
- Introduce students to a range of high-throughput experimental methods currently being used in biochemistry, molecular biology and genetics.
- Make students aware of data analysis methods and issues associated with the interpretation of high-throughput data.
- Allow students to appreciate the applications of high-throughput data generation to advance bioscience and medicine.

Learning outcomes
On completion of the module, students should be able to:
1. Know of a range of currently important experimental methods which generate high-throughput data in molecular biology and genetics;
2. Explain how these high-throughput methods work;
3. Demonstrate an understanding of the specific and generic data analysis methods and issues associated with high throughput data;
4. Describe how high-throughput data can advance bioscience and medicine;
5. Analyse and interpret high-throughput data; and
6. Critically evaluate relevant research papers.

Syllabus

High-throughput (new/next generation) DNA sequencing, and applications in genome sequencing (genetics, cancer, rare diseases), epigenetics and genetic regulation (methylation, histone modification, DNA accessibility, ChIP-seq), gene expression studies (RNA-seq), studies of other RNA species (miRNA, lncRNA, circle RNA) and translation.
High-throughput proteomics and metabolomics using mass spectrometry, NMR and other methods. Chemical proteomics, high-throughput analysis of protein interactions.
High-throughput phenotypic screens, whole or part genome siRNA screens. Phenotypic screens using small molecule libraries.
High-throughput methods in structural biology and drug/fragment screening.

Teaching methods

Delivery type	Number	Length hours	Student hours
Practical	3	3.00	9.00
Seminar	6	3.00	18.00
Private study hours			123.00
Total Contact hours			27.00
Total hours (100hr per 10 credits)			150.00

Private study

Private study will be preparation for the seminars and work on the assessments.

Opportunities for Formative Feedback

Seminars will occur weekly and progress monitored during the sessions through the discussions that will take place. Practicals will occur fortnightly and progress monitored during these sessions through completion of the formative tasks.

Methods of assessment

Coursework

Assessment type	Notes	% of formal assessment
Essay	2,500 word report	60.00
Presentation	Verbal presentation (10 minutes plus 5 minutes questions)	40.00
Practical	Formative assessment	0.00
Total percentage (Assessment Coursework)		100.00

Resits will be as per the original coursework.

Reading list

The reading list is available from the Library website

Last updated: 20/05/2019

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