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2019/20 Taught Postgraduate Module Catalogue

BIOL5112M Bioimaging

10 creditsClass Size: 24

Module manager: Dr Sally Boxall
Email: S.A.Boxall@leeds.ac.uk

Taught: Semester 1 View Timetable

Year running 2019/20

Pre-requisite qualifications

BSc in Biological Sciences and knowledge of animal cell biology or pre-requisite modules.

This module is mutually exclusive with

BIOL5312MBioimaging

This module is not approved as an Elective

Objectives

Learning outcomes
On completion of this module, students should be able to:
- Understand the principles of phase, DIC, fluorescence, deconvolution and confocal microscopy, calcium imaging, and transmission and scanning electron microscopy;
- Be able to perform the techniques required to obtain epifluorescence, confocal and deconvolution images of fluorescently stained fixed cells;
- Explain how to image live cells with an epiflourescent or confocal microscope;
- Understand the principles of the techniques FRAP, FRET and FLIM microscopy;
- Understand the principles of calcium imaging in live cells;
- Understand the principles of imaging cells, and single molecules in the electron microscope.

Skills outcomes
- This module will provide technical training in practical microscopy and opportunities to generate andanalyse images. - - Technical writing skills will be developed through production of a practical report and an essay on a topic related to bioimaging


Syllabus

Bio-imaging encompasses a wide range of approaches from imaging single molecules in the electron
microscope, to determining the localisation of proteins in fixed cells by fluorescence microscopy. Any
one, or a combination of techniques can build up a picture of the structure and function of a protein of
interest and its biological role in the cell.

The aim of this module is to provide an introduction to the wide range of approaches used in Bioimaging and their relative advantages and disadvantages for analysing protein and cellular function.

The module will include a range of lectures/seminars on the techniques/approaches involved together
with practical demonstrations and practical work to enable the student to obtain hands on experience
of some of the techniques. The practical work will be based on visualising the cellular cytoskeleton
(actin, intermediate filaments and microtubules) and molecular motors (myosin, kinesin) and investigating organelle dynamics and protein localisation in plant cells.

Teaching methods

Delivery typeNumberLength hoursStudent hours
Lecture62.0012.00
Practical63.0018.00
Private study hours70.00
Total Contact hours30.00
Total hours (100hr per 10 credits)100.00

Private study

- 3 hours reading for each 2 hour tutorial: 18 hours;
- 2 hours reading for each 1 hour tutorial: 4 hours;
- Preparation of 2 x 1000 word essays: 22 hours;
- Reading for practical work, and preparation of practical report: 26 hours.

Opportunities for Formative Feedback

1 x Practical report and 1 x Essay. Written feedback and oral comments in tutorial sessions and practical classes.

Methods of assessment


Coursework
Assessment typeNotes% of formal assessment
Practical Report2500 words50.00
Essay2000 words50.00
Total percentage (Assessment Coursework)100.00

Normally resits will be assessed by the same methodology as the first attempt, unless otherwise stated

Reading list

The reading list is available from the Library website

Last updated: 30/04/2019

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