2022/23 Undergraduate Module Catalogue
BLGY1234 Practical Genetics
10 creditsClass Size: 140
Module manager: Dr Christopher West
Taught: Semester 2 (Jan to Jun) View Timetable
Year running 2022/23
Pre-requisite qualificationsBiology A level or equivalent
This module is not approved as a discovery module
Module summaryDNA isolation, PCR and restriction digests are fundamental analytical techniques and used in molecular cloning. This module will provide hands on experience of these core techniques, in addition to applying the theory of Mendelian genetics, cytology and the principles of genetic linkage to real-world genetics problems.
ObjectivesAn understanding of the experimental techniques that underpin knowledge of genetic mechanisms in living organisms.
On completion of the module students will be able to:
- demonstrate the principles of genetic segregation;
- provide experience of genetic analysis in bacteria and eukaryotes;
- use techniques used in molecular genetic manipulation and analysis;
- explain how PCR works and its applications in molecular biology.
- Good Laboratory Practice;
- Core molecular biology techniques.
This module will provide core genetics practical skills to support the Introduction to Genetics module. This will include phenotypic analysis to provide an understanding of Mendelian segregation. Molecular skills will be provided through experience of DNA isolation, restriction analysis, agarose gel electrophoresis and recombinant DNA technology (molecular cloning). Students will analyse purified DNA by PCR for the detection of transgenes. PCR will also be used to track a mutant allele linked to a CAPS marker (cleaved amplified polymorphic sequence). This will allow students to predict disease pre-disposition in a family. In the final practical students will learn cytological methods to identify genetic disease.
|Delivery type||Number||Length hours||Student hours|
|Private study hours||69.00|
|Total Contact hours||31.00|
|Total hours (100hr per 10 credits)||100.00|
Private studyPrivate study will include completion of coursework elements (30 hours), background reading (39 hours) in support of the practicals (reading handouts and associated literature) and in researching coursework elements.
Opportunities for Formative FeedbackFeedback is provided by staff and demonstrators during the practical as progress is monitored. Feedback for the MCQ-assessed practicals is provided online. The practical report is annotated and returned to students.
Methods of assessment
|Assessment type||Notes||% of formal assessment|
|In-course MCQ||MCQ test||30.00|
|Total percentage (Assessment Coursework)||100.00|
Normally resits will be assessed by the same methodology as the first attempt, unless otherwise stated
Reading listThe reading list is available from the Library website
Last updated: 09/12/2022
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